Application
Fig1: Western blot analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVECApplication
Fig2: ICC staining THY1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining THY1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-THY1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. Counter stained with hematoxylin.| Product Name | THY1 [JF10-09] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD7 antibody CD90 antibody CD90 antigen antibody CDw90 antibody FLJ33325 antibody MGC128895 antibody T25 antibody Theta antigen antibody Thy 1 antibody Thy 1 cell surface antigen antibody Thy 1 membrane glycoprotein antibody Thy 1 T cell antigen antibody Thy 1.2 antibody Thy-1 antigen antibody Thy-1 membrane glycoprotein antibody Thy1 antibody Thy1 antigen antibody Thy1 T cell antigen antibody Thy1.1 antibody Thy1.2 antibody THY1_HUMAN antibody Thymus cell antigen 1, theta antibody |
| Product description | Over 100 cell surface markers have been identified through the use of monoclonal antibodies. Many of these markers have proven useful in identifying specific subpopulations of cells within mixed colonies. Accordingly, these molecules have been assigned a "cluster of differentiation" (CD) designation. One such marker, designated Thy-1 (also referred to as CDw90), is a phosphatidyl-anchored cell surface glycoprotein which, when coexpressed with CD34 on cells from normal human bone marrow, identifies a subpopulation that includes putative hematopoietic, pleuripotent stem cells. Thy-1+ cells from bone marrow have been implicated in syngeneic graft versus host disease and may serve to regulate autoreactivity after bone marrow transplant. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD7 antibody
CD90 antibody
CD90 antigen antibody
CDw90 antibody
FLJ33325 antibody
MGC128895 antibody
T25 antibody
Theta antigen antibody
Thy 1 antibody
Thy 1 cell surface antigen antibody
Thy 1 membrane glycoprotein antibody
Thy 1 T cell antigen antibody
Thy 1.2 antibody
Thy-1 antigen antibody
Thy-1 membrane glycoprotein antibody
Thy1 antibody
Thy1 antigen antibody
Thy1 T cell antigen antibody
Thy1.1 antibody
Thy1.2 antibody
THY1_HUMAN antibody
Thymus cell antigen 1 theta antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P04216 |
|---|
Application
Fig1: Western blot analysis of THY1 on different lysates using anti-THY1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: HUVEC
Application
Fig2: ICC staining THY1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining THY1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining THY1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-THY1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. Counter stained with hematoxylin.| Positive Control | HUVEC, MCF-7, Hela, HepG2, PC-12, human tonsil tissue, human lung cancer tissue, human spleen tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
