Application
Fig1: Western blot analysis of ADAM17 on Jurkat cells lysates using anti-ADAM17 antibody at 1/1,000 dilution.Application
Fig2: ICC staining ADAM17 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ADAM17 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ADAM17 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with ADAM17 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ADAM17 [JM10-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody A disintegrin and metalloproteinase domain 17 antibody ADA17_HUMAN antibody ADAM 17 antibody ADAM metallopeptidase domain 17 antibody ADAM17 antibody ADAM17 protein antibody CD 156b antibody CD156b antibody CD156b antigen antibody CSVP antibody Disintegrin and metalloproteinase domain-containing protein 17 antibody MGC71942 antibody NISBD antibody NISBD1 antibody Snake venom like protease antibody Snake venom-like protease antibody TACE antibody TNF alpha convertase antibody TNF alpha converting enzyme antibody TNF-alpha convertase antibody TNF-alpha-converting enzyme antibody Tumor Necrosis Factor Alpha Converting Enzyme antibody |
| Product description | Tumor necrosis factor β (TNFβ), also known as lymphotoxin, is a pleiotropic cytokine. TNFα, also known as cachetin, is a cytokine that binds to the same receptors, producing an array of effects similar to those of TNFβ. TNFβ and TNFα share 30% amino acid homology and have similar biological activities. TNFβ is produced by activated lymphocytes, including CD4+ T helper cell type 1 lymphocytes, CD8+ lymphocytes and certain B lymphoblastoid cell lines. TNFα is produced by several different cell types, including lymphocytes, neutrophils and macrophages. TNFβ and TNFα can modulate many immune and inflammatory functions while having the ability to inhibit tumor growth. TACE (for TNFα converting enzyme) is a metalloproteinase that cleaves the membrane-bound TNFα precursor to release soluble TNFα. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIPFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:50-1:200 IP:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | A disintegrin and metalloproteinase domain 17 (tumor necrosis factor alpha converting enzyme) antibody A disintegrin and metalloproteinase domain 17 antibody ADA17_HUMAN antibody ADAM 17 antibody ADAM metallopeptidase domain 17 antibody ADAM17 antibody ADAM17 protein antibody CD 156b antibody CD156b antibody CD156b antigen antibody CSVP antibody Disintegrin and metalloproteinase domain-containing protein 17 antibody MGC71942 antibody NISBD antibody NISBD1 antibody Snake venom like protease antibody Snake venom-like protease antibody TACE antibody TNF alpha convertase antibody TNF alpha converting enzyme antibody TNF-alpha convertase antibody TNF-alpha-converting enzyme antibody Tumor Necrosis Factor Alpha Converting Enzyme antibody |
|---|---|
| Molecular Weight(MW) | 93 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of ADAM17 on Jurkat cells lysates using anti-ADAM17 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining ADAM17 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ADAM17 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ADAM17 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with ADAM17 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, SKOV-3, SW480, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 ICC:1:50-1:200 IP:1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
