Application
Fig1: Western blot analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1,000 dilution.Application
Fig2: ICC staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining K63-linkage Specific Ubiquitin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining K63-linkage Specific Ubiquitin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.| Product Name | K63-linkage Specific Ubiquitin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S Proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lys 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκB-α and p27. |
| Immunogen | recombinant protein |
| Isotype | IgG |
|---|---|
| Host Species | Recombinant rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Epididymis secretory protein Li 50 antibody FLJ25987 antibody HEL S 50 antibody MGC8385 antibody Polyubiquitin B antibody RPS 27A antibody RPS27A antibody UBA 52 antibody UBA 80 antibody UBA52 antibody UBA80 antibody UBB antibody UBB_HUMAN antibody UBC antibody UBCEP 1 antibody UBCEP 2 antibody UBCEP1 antibody UBCEP2 antibody Ubiquitin antibody Ubiquitin B antibody |
|---|---|
| Molecular Weight(MW) | 60-100 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining K63-linkage Specific Ubiquitin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining K63-linkage Specific Ubiquitin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.| Positive Control | 293T, N2A, Hela, human liver cancer tissue, human colon cancer tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
