Application
Fig1: Western blot analysis of AhR on human lung lysates using anti-AhR antibody at 1/1,000 dilution.Application
Fig2: ICC staining AhR in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining AhR in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining AhR in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Aryl hydrocarbon Receptor [JM34-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is the prototype for a family of toxic halogenated aromatic compounds that are thought to cause adverse reproductive, immunologic and metabolic effects. Many biological responses to TCDD are mediated through ligand binding to the aromatic hydrocarbon (Ah) receptor, also known as AhR. Ah Receptor is a ligand dependent transcription factor that interacts with specific DNA sequences, termed xenobiotic responsive elements (XREs), and that lies upstream of TCDD-inducible genes. Upon binding to the ligand, Ah Receptor binds to the Ah Receptor nuclear translocator (Arnt), and the complex is translocated from the cytoplasm to the nucleus. Arnt is required for Ah R eceptor to bind to XRE. Ah Receptor and Arnt are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common "PAS" motif. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Ah receptor antibody AhR antibody AHR_HUMAN antibody Aromatic hydrocarbon receptor antibody Aryl hydrocarbon receptor antibody Aryl hydrocarbon receptor precursor antibody bHLHe76 antibody Class E basic helix loop helix protein 76 antibody Class E basic helix-loop-helix protein 76 antibody HGNC:348 antibody |
|---|---|
| Molecular Weight(MW) | 96 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P35869 |
|---|
Application
Fig1: Western blot analysis of AhR on human lung lysates using anti-AhR antibody at 1/1,000 dilution.
Application
Fig2: ICC staining AhR in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining AhR in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining AhR in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | NIH/3T3, MCF-7, Hela, human lung tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
