Application
Fig3: Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig3: Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig3: Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | NG2 [JM10-13] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4732461B14Rik antibody AN2 antibody AN2 proteoglycan antibody Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody Chondroitin sulfate proteoglycan 4 antibody Chondroitin sulfate proteoglycan NG2 antibody Cspg4 antibody Cspg4 chondroitin sulfate proteoglycan 4 antibody CSPG4_HUMAN antibody HMW-MAA antibody HSN tumor-specific antigen antibody Kiaa4232 antibody MCSP antibody MCSPG antibody MEL-CSPG antibody Melanoma chondroitin sulfate proteoglycan antibody Melanoma-associated chondroitin sulfate proteoglycan antibody MELCSPG antibody MSK16 antibody NG2 antibody |
| Product description | NG2 (also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16 and MEL-CSPG) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (Threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with α4 β1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Microvessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 4732461B14Rik antibody AN2 antibody AN2 proteoglycan antibody Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody Chondroitin sulfate proteoglycan 4 antibody Chondroitin sulfate proteoglycan NG2 antibody Cspg4 antibody Cspg4 chondroitin sulfate proteoglycan 4 antibody CSPG4_HUMAN antibody HMW-MAA antibody HSN tumor-specific antigen antibody Kiaa4232 antibody MCSP antibody MCSPG antibody MEL-CSPG antibody Melanoma chondroitin sulfate proteoglycan antibody Melanoma-associated chondroitin sulfate proteoglycan antibody MELCSPG antibody MSK16 antibody NG2 antibody |
|---|---|
| Molecular Weight(MW) | 251 kDa |
| Cellular Localization | Cell membrane, Apical cell membrane, Cell projection, Cell surface. |
| SwissProt ID | Q6UVK1 |
|---|
Application
Fig3: Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig3: Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig3: Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Human kidney tissue, rat brain tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
