Application
Fig1: Western blot analysis of Myeloperoxidase on HL-60 cell lysates using anti-Myeloperoxidase antibody at 1/1,000 dilution.Application
Fig2: ICC staining Myeloperoxidase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Myeloperoxidase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Myeloperoxidase in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.| Product Name | Myeloperoxidase [JM10-58] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 84 kDa myeloperoxidase antibody 89 kDa myeloperoxidase antibody EC 1.11.1.7 antibody EC1.11.2.2 antibody fj80f04 antibody MPO antibody mpx antibody myeloid-specific peroxidase antibody Myeloperoxidase antibody Myeloperoxidase heavy chain antibody Myeloperoxidase light chain antibody PERM_HUMAN antibody wu:fj80f04 antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa |
| Cellular Localization | Lysosome. |
| SwissProt ID | P05164 |
|---|
Application
Fig1: Western blot analysis of Myeloperoxidase on HL-60 cell lysates using anti-Myeloperoxidase antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Myeloperoxidase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Myeloperoxidase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Myeloperoxidase in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.| Positive Control | HL-60, AGS, Hela, MCF-7, human lung tissue, human spleen tissue, human tonsil tissue, mouse brain tissue, mouse spinal cord tissue, mouse colon tissue, mouse skin tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
