Application
Fig1: Western blot analysis of PGP9.5 on A549 cell lysates using anti-PGP9.5 antibody at 1/1,000 dilution.Application
Fig2: ICC staining PGP9.5 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PGP9.5 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.| Product Name | PGP9.5 [JM10-59] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | UCH-L1 (ubiquitin C-terminal hydrolase) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1-2% of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p13. The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC,IP |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 IP:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | Epididymis luminal protein 117 antibody Epididymis secretory protein Li 53 antibody HEL 117 antibody HEL S 53 antibody NDGOA antibody Neuron cytoplasmic protein 9.5 antibody OTTHUMP00000218137 antibody OTTHUMP00000218139 antibody OTTHUMP00000218140 antibody OTTHUMP00000218141 antibody Park 5 antibody PARK5 antibody PGP 9.5 antibody PGP9.5 antibody PGP95 antibody Protein gene product 9.5 antibody Ubiquitin C terminal esterase L1 antibody Ubiquitin C terminal hydrolase antibody Ubiquitin C terminal hydrolase L1 antibody Ubiquitin carboxyl terminal esterase L1 antibody Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody Ubiquitin thioesterase L1 antibody Ubiquitin thiolesterase antibody Ubiquitin thiolesterase L1 antibody UCH-L1 antibody UCHL1 antibody UCHL1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum membrane. |
Application
Fig1: Western blot analysis of PGP9.5 on A549 cell lysates using anti-PGP9.5 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining PGP9.5 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PGP9.5 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.| Positive Control | A549, N2A, PC-12, human pancreas tissue, mouse brain tissue, mouse spinal cord tissue, mouse colon tissue, mouse skin tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 IP:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
