Application
Fig1: Western blot analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.Application
Fig2: ICC staining SDHA in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining SDHA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining SDHA in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHA antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHA antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SDHA antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SDHA antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHA antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SDHA antibody. Counter stained with hematoxylin.Application
Fig11: Flow cytometric analysis of Hela cells with SDHA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | SDHA [JM10-83] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMD1GG antibody DHSA_HUMAN antibody Flavoprotein subunit of complex II antibody Fp antibody PGL5 antibody SDH 2 antibody SDH1 antibody SDH2 antibody SDHA antibody SDHF antibody Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody Succinate dehydrogenase complex flavoprotein subunit A antibody Succinate dehydrogenase complex flavoprotein subunit antibody Succinate dehydrogenase complex flavoprotein subunit precursor antibody Succinate dehydrogenase complex subunit A antibody Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody Succinate dehydrogenase complex subunit A flavoprotein antibody |
| Product description | In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | CMD1GG antibody
DHSA_HUMAN antibody
Flavoprotein subunit of complex II antibody
Fp antibody
PGL5 antibody
SDH 2 antibody
SDH1 antibody
SDH2 antibody
SDHA antibody
SDHF antibody
Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody
Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody
Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody Succinate dehydrogenase complex flavoprotein subunit A antibody Succinate dehydrogenase complex flavoprotein subunit antibody Succinate dehydrogenase complex flavoprotein subunit precursor antibody Succinate dehydrogenase complex subunit A antibody Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody Succinate dehydrogenase complex subunit A flavoprotein antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Mitochondrion inner membrane. |
Application
Fig1: Western blot analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.
Application
Fig2: ICC staining SDHA in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining SDHA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining SDHA in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHA antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHA antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SDHA antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SDHA antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHA antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SDHA antibody. Counter stained with hematoxylin.
Application
Fig11: Flow cytometric analysis of Hela cells with SDHA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, MCF-7, HepG2, NIH-3T3, Hela, human liver tissue, human kidney tissue, mouse testis tissue, mouse skeletal muscle tissue, mouse colon tissue, zebrafish tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
