Application
Fig1: Western blot analysis of Syndecan 1 on NIH-3T3 cells lysates using anti-Syndecan 1 antibody at 1/500 dilution.Application
Fig2: ICC staining Syndecan 1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Syndecan 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Syndecan 1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.| Product Name | Syndecan 1 [JM11-21] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Syndecan-1 (SYND1), also designated CD138, is a type I integral membrane proteoglycan that contains both chondroitin sulfate and heparan sulfate groups. It is expressed in mouse on pre-B cells, immature B cells and plasma cells. Syndecan-1 is also found on the basolateral surfaces of epithelial cells, endothelial cells of sprouting capillaries and embryonic condensing mesenchymal cells. Syndecan-1 functions as an extracellular matrix receptor which binds to collagens, Fibronectin and Thrombospondin. It has been shown to co-localize with Actin-rich filaments and may act to link the cytoskeleton to the extracellular matrix. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | CD_antigen antibody CD138 antibody CD138 antigen antibody heparan sulfate proteoglycan fibroblast growth factor receptor antibody SDC antibody Sdc1 antibody SDC1_HUMAN antibody SYND1 antibody Syndecan 1 antibody Syndecan antibody syndecan proteoglycan 1 antibody Syndecan-1 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of Syndecan 1 on NIH-3T3 cells lysates using anti-Syndecan 1 antibody at 1/500 dilution.
Application
Fig2: ICC staining Syndecan 1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Syndecan 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Syndecan 1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.| Positive Control | A431, HepG2, NIH-3T3, Hela, human tonsil tissue, human kidney tissue, human lung tissue, mouse prostate tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
