Application
Fig1: Western blot analysis of CYP2E1 on human liver cells lysates using anti-CYP2E1 antibody at 1/500 dilution.Application
Fig2: ICC staining CYP2E1 in 293-T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CYP2E1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.| Product Name | CYP2E1 [JM10-85] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,ICC/IF,IHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | 4-nitrophenol 2-hydroxylase antibody
CP2E1_HUMAN antibody
CPE1 antibody
CYP2E antibody
Cyp2e1 antibody
CYPIIE1 antibody
Cytochrome P450 2E1 antibody
Cytochrome P450 isozyme 3A antibody
Cytochrome P450 LM3A antibody
Cytochrome P450 family 2 subfamily e polypeptide 1 antibody cytochrome P450 subfamily IIE (ethanol-inducible) polypeptide 1 antibody Cytochrome P450 subfamily IIE antibody Cytochrome P450-ALC antibody Cytochrome P450-J antibody Cytochrome P450RLM6 antibody EC 1.14.13.- antibody EC 1.14.13.n7 antibody Ethanol-inducible P450 antibody Flavoprotein-linked monooxygenase antibody MGC133529 antibody Microsomal monooxygenase antibody OTTHUMP00000020813 antibody OTTHUMP00000046571 antibody P450 ALC antibody P450-J antibody P450C2E antibody P450J antibody P450RLM6 antibody Xenobiotic monooxygenase antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. Microsome membrane. |
Application
Fig1: Western blot analysis of CYP2E1 on human liver cells lysates using anti-CYP2E1 antibody at 1/500 dilution.
Application
Fig2: ICC staining CYP2E1 in 293-T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CYP2E1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-CYP2E1 antibody. Counter stained with hematoxylin.| Positive Control | 293-T, Hela, human liver cancer tissue, human liver tissue, mouse liver tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
