Application
Fig1: Western blot analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.Application
Fig2: Flow cytometric analysis of SH-SY5Y cells with CaMKII alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CaMKII alpha [JM11-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha CaMKII antibody Calcium calmodulin dependent protein kinase II antibody Calcium/calmodulin dependent protein kinase II alpha B subunit antibody Calcium/calmodulin dependent protein kinase type II alpha chain antibody Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody Calcium/calmodulin-dependent protein kinase II alpha antibody Calcium/calmodulin-dependent protein kinase II-alpha antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody Calcium/calmodulin-dependent protein kinase type IIA antibody CaM kinase II alpha chain antibody CaM kinase II alpha subunit antibody CaM kinase II subunit alpha antibody CaMK II alpha subunit antibody CaMK-II subunit alpha antibody Camk2a antibody CAMKA antibody CaMKII antibody CaMKIINalpha antibody EC 2.7.11.17 antibody KCC2A_HUMAN antibody KIAA0968 antibody MGC123320 antibody MGC139375 antibody MGC155201 antibody mKIAA0968 antibody PK2CDD antibody PKCCD antibody R74975 antibody zgc:112538 antibody zgc:123320 antibody |
| Product description | dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:1,000-1:2,000 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alpha CaMKII antibody Calcium calmodulin dependent protein kinase II antibody Calcium/calmodulin dependent protein kinase II alpha B subunit antibody Calcium/calmodulin dependent protein kinase type II alpha chain antibody Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody Calcium/calmodulin-dependent protein kinase II alpha antibody Calcium/calmodulin-dependent protein kinase II-alpha antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody Calcium/calmodulin-dependent protein kinase type IIA antibody CaM kinase II alpha chain antibody CaM kinase II alpha subunit antibody CaM kinase II subunit alpha antibody CaMK II alpha subunit antibody CaMK-II subunit alpha antibody Camk2a antibody CAMKA antibody CaMKII antibody CaMKIINalpha antibody EC 2.7.11.17 antibody KCC2A_HUMAN antibody KIAA0968 antibody MGC123320 antibody MGC139375 antibody MGC155201 antibody mKIAA0968 antibody PK2CDD antibody PKCCD antibody R74975 antibody zgc:112538 antibody zgc:123320 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cell junction, synapse, presynaptic cell membrane. |
Application
Fig1: Western blot analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.
Application
Fig2: Flow cytometric analysis of SH-SY5Y cells with CaMKII alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | SH-SY5Y, rat brain cells. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
