WB
Western blot analysis of CD40L on different lysates with Rabbit anti-CD40L antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane), Lane 2: K-562 cell lysate (15 µg/Lane), Lane 3: PC-12 cell lysate (15 µg/Lane), Lane 4: Mouse liver tissue lysate (20 µg/Lane), Lane 5: Rat thymus tissue lysate (20 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | CD40L Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. |
| Immunogen | Synthetic peptide within Human CD40L aa 103-150 / 261. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | CD 40L antibody
CD154 antibody
CD40 antigen ligand antibody
CD40 ligand antibody
CD40 ligand soluble form antibody CD40-L antibody CD40L antibody CD40L_HUMAN antibody CD40LG antibody gp39 antibody hCD40L antibody HIGM1 antibody IGM antibody IMD3 antibody T B cell activating molecule antibody T BAM antibody T-cell antigen Gp39 antibody TNF-related activation protein antibody TNFSF5 antibody TrAP antibody Tumor necrosis factor (ligand) superfamily member 5 antibody Tumor necrosis factor ligand superfamily member 5 antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa(Observed band size: 40 kDa) |
| Cellular Localization | Cell membrane, Cell surface, Secreted. |
WB
Western blot analysis of CD40L on different lysates with Rabbit anti-CD40L antibody at 1/5,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane), Lane 2: K-562 cell lysate (15 µg/Lane), Lane 3: PC-12 cell lysate (15 µg/Lane), Lane 4: Mouse liver tissue lysate (20 µg/Lane), Lane 5: Rat thymus tissue lysate (20 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
