Application
Fig1: Western blot analysis of FMRP on different cells lysates using anti-FMRP antibody at 1/500 dilution. Positive control： Lane 1: Hela Lane 2: K562Application
Fig2: ICC staining FMRP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining FMRP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining FMRP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FMRP antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-FMRP antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-FMRP antibody. Counter stained with hematoxylin.| Product Name | FMRP [JM91-41] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FMR 1 antibody Fmr1 antibody Fmr1 gene antibody FMR1_HUMAN antibody FMRP antibody Fragile X mental retardation 1 antibody Fragile X mental retardation 1 protein antibody Fragile X mental retardation protein 1 antibody Fragile X mental retardation protein antibody fragile X mental retardation syndrome-related protein 1 antibody fragile X mental retardation, autosomal homolog 1 antibody FRAXA antibody fxr1 antibody MGC87458 antibody POF antibody POF1 antibody Protein FMR-1 antibody Protein FMR1 antibody wu:fb16f11 antibody wu:fd18c10 antibody zgc:66226 antibody |
| Product description | Fragile X syndrome is the most frequent form of inherited mental retardation and is the result of transcriptional silencing of the FMR1 gene on the X chromosome. The FMR1 gene contains a distinct CpG dinucleotide repeat located in the 5' untranslated region of the gene. In fragile X syndrome this tandem repeat is substantially amplified and subjected to extensive methylation and enhanced transcriptional silencing. The FMR1 protein (or FMRP) is an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. It contains several features that are characteristics of RNA-binding proteins, including two hnRNPK homology (KH) domains and an RGG amino acid motif (RGG box). FMR1 localizes to both the nucleus and the cytoplasm and can also interact with two fragile X syndrome related factors, FXR1 and FXR2, which form heterodimers through their N-terminal coiled-coil domains. Since FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in the nucleocytoplasmic transport of mRNAs. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:5,000-1:10,000 ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FMR 1 antibody
Fmr1 antibody
Fmr1 gene antibody
FMR1_HUMAN antibody
FMRP antibody
Fragile X mental retardation 1 antibody
Fragile X mental retardation 1 protein antibody
Fragile X mental retardation protein 1 antibody
Fragile X mental retardation protein antibody
fragile X mental retardation syndrome-related protein 1 antibody
fragile X mental retardation autosomal homolog 1 antibody FRAXA antibody fxr1 antibody MGC87458 antibody POF antibody POF1 antibody Protein FMR-1 antibody Protein FMR1 antibody wu:fb16f11 antibody wu:fd18c10 antibody zgc:66226 antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of FMRP on different cells lysates using anti-FMRP antibody at 1/500 dilution. Positive control： Lane 1: Hela Lane 2: K562
Application
Fig2: ICC staining FMRP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining FMRP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining FMRP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FMRP antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-FMRP antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-FMRP antibody. Counter stained with hematoxylin.| Positive Control | Hela, HepG2, K562, SH-SY5Y, human tonsil tissue, mouse colon tissue, rat brain tissue. |
|---|---|
| Application Notes | WB:1:5,000-1:10,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
