Application
Fig1: Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- S100 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- S100 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-S100 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of SH-SY5Y cells with S100 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondar antibody.| Product Name | S100 [JM51-31] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEF antibody Protein S100 B antibody Protein S100-B antibody S 100 calcium binding protein beta chain antibody S 100 protein beta chain antibody S-100 protein beta chain antibody S-100 protein subunit beta antibody S100 antibody S100 calcium binding protein beta (neural) antibody S100 calcium-binding protein B antibody S100 protein beta chain antibody S100B antibody S100B_HUMAN antibody S100beta antibody |
| Product description | The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | NEF antibody Protein S100 B antibody Protein S100-B antibody S 100 calcium binding protein beta chain antibody S 100 protein beta chain antibody S-100 protein beta chain antibody S-100 protein subunit beta antibody S100 antibody S100 calcium binding protein beta (neural) antibody S100 calcium-binding protein B antibody S100 protein beta chain antibody S100B antibody S100B_HUMAN antibody S100beta antibody |
|---|---|
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- S100 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- S100 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-S100 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of SH-SY5Y cells with S100 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondar antibody.| Positive Control | Mouse heart, SH-SY5Y, human tonsil tissue, human kidney tissue, mouse brain tissue, mouse spinal cord tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
