Application
Fig1: Western blot analysis of TDP43 on different cells lysates using anti-TDP43 antibody at 1/500 dilution. Positive control： Line 1: Hela Line 2: K563Application
Fig2: ICC staining TDP43 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining TDP43 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining TDP43 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP43 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TDP43 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TDP43 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with TDP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | TDP43 [JM51-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB,IP,ICC/IF,IHC,FC |
| WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | Human,Mouse,Rat,Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | ALS10 antibody OTTHUMP00000002171 antibody OTTHUMP00000002172 antibody OTTHUMP00000002173 antibody TADBP_HUMAN antibody TAR DNA binding protein 43 antibody TAR DNA binding protein antibody TAR DNA-binding protein 43 antibody TARDBP antibody TDP 43 antibody TDP-43 antibody TDP43 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Nucleus.. |
Application
Fig1: Western blot analysis of TDP43 on different cells lysates using anti-TDP43 antibody at 1/500 dilution. Positive control： Line 1: Hela Line 2: K563
Application
Fig2: ICC staining TDP43 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TDP43 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining TDP43 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with TDP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, K563, 293T, Hela, SH-SY5Y, human spleen tissue, human pancreas tissue, mouse placenta tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
