Application
Fig1: Western blot analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control： Line 1: MCF-7 Line 2: A549Application
Fig2: ICC staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | NMDAR1 [JM11-26] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GluN1 antibody Glutamate [NMDA] receptor subunit zeta-1 antibody Glutamate receptor ionotropic N methyl D aspartate 1 antibody Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody glutamate receptor ionotropic, NMDA 1 antibody Grin1 antibody MRD8 antibody N methyl D aspartate receptor antibody N methyl D aspartate receptor channel subunit zeta 1 antibody N methyl D aspartate receptor subunit NR1 antibody N-methyl-D-aspartate receptor subunit NR1 antibody NMD-R1 antibody NMDA 1 antibody NMDA R1 antibody NMDA receptor 1 antibody NMDA1 antibody NMDAR antibody NMDZ1_HUMAN antibody NR1 antibody |
| Product description | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | GluN1 antibody
Glutamate [NMDA] receptor subunit zeta-1 antibody
Glutamate receptor ionotropic N methyl D aspartate 1 antibody
Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody glutamate receptor ionotropic NMDA 1 antibody Grin1 antibody MRD8 antibody N methyl D aspartate receptor antibody N methyl D aspartate receptor channel subunit zeta 1 antibody N methyl D aspartate receptor subunit NR1 antibody N-methyl-D-aspartate receptor subunit NR1 antibody NMD-R1 antibody NMDA 1 antibody NMDA R1 antibody NMDA receptor 1 antibody NMDA1 antibody NMDAR antibody NMDZ1_HUMAN antibody NR1 antibody |
|---|---|
| Molecular Weight(MW) | 105 kDa |
| Cellular Localization | Cell membrane. Cell junction. |
Application
Fig1: Western blot analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control： Line 1: MCF-7 Line 2: A549
Application
Fig2: ICC staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, A549, N2A, SH-SY5Y, SHG-44, mouse brain tissue, rat brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
