Fig1: Western blot analysis of Aromatase on different cells lysates using anti-Aromatase antibody at 1/500 dilution. Positive control: Lane 1: JAR Lane 2: Jurkat
r P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased CYP19 activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7α-hydroxylase, CYP7A1, is the rate-limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver.
Fig1: Western blot analysis of Aromatase on different cells lysates using anti-Aromatase antibody at 1/500 dilution. Positive control: Lane 1: JAR Lane 2: Jurkat
Positive Control
JAR, Jurkat.
Application Notes
WB:1:500-1:2,000 IP:1:10-1:50
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.