Application
Fig1: Western blot analysis of TIMP2 on K562 cells lysates using anti-TIMP2 antibody at 1/500 dilution.Application
Fig2: Flow cytometric analysis of Hela cells with TIMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | TIMP2 [JM87-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSC 21K antibody CSC-21K antibody CSC21K antibody Metalloproteinase inhibitor 2 antibody Metalloproteinase inhibitor 2 precursor antibody TIMP 2 antibody TIMP metallopeptidase inhibitor 2 antibody TIMP-2 antibody TIMP2 antibody TIMP2_HUMAN antibody Tissue Inhibitor of Metalloproteinase 2 antibody Tissue inhibitor of metalloproteinases 2 antibody |
| Product description | TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases 1, 2, 3 and 4) complex with metalloproteinases such as collagenases, gelatin-ases and stromelysins, resulting in irreversible inactivation of the metallopro-teinase. TIMP-1 has been found to be identical to EPA (erythroid-potentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus and remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart, with low levels expressed in liver, brain, lung, thymus and spleen. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:10-1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CSC 21K antibody CSC-21K antibody CSC21K antibody Metalloproteinase inhibitor 2 antibody Metalloproteinase inhibitor 2 precursor antibody TIMP 2 antibody TIMP metallopeptidase inhibitor 2 antibody TIMP-2 antibody TIMP2 antibody TIMP2_HUMAN antibody Tissue Inhibitor of Metalloproteinase 2 antibody Tissue inhibitor of metalloproteinases 2 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P16035 |
|---|
Application
Fig1: Western blot analysis of TIMP2 on K562 cells lysates using anti-TIMP2 antibody at 1/500 dilution.
Application
Fig2: Flow cytometric analysis of Hela cells with TIMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | K562, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
