Application
Fig1: Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.| Product Name | RPE65 [JM61-51] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | All-trans-retinyl-palmitate hydrolase antibody LCA 2 antibody LCA2 antibody Leber congenital amaurosis antibody mRPE 65 antibody mRPE65 antibody p63 antibody rd 12 antibody rd12 antibody Retinal pigment epithelium specific 61 kDa protein antibody Retinal pigment epithelium specific 65 kDa protein antibody Retinal pigment epithelium specific protein antibody Retinal pigment epithelium specific protein 65kDa antibody Retinal pigment epithelium-specific 65 kDa protein antibody Retinitis pigmentosa 20 antibody Retinoid isomerohydrolase antibody Retinol isomerase antibody RP 20 antibody RP20 antibody RPE 65 antibody RPE65 antibody RPE65_HUMAN antibody sRPE 65 antibody sRPE65 antibody |
| Product description | The retinal pigment epithelium (RPE) is a monolayer simple epithelium in proximity to the outer surface of the retinal photoreceptor cells. Retinal pigment epithelium-specific protein (RPE65) is a 65kDa protein belonging to the beta-carotene dioxygenase family. This protein is important in 11-cis retinal production as well as in visual pigment regeneration. RPE65 is attached to the membrane by a lipid anchor when palmitoylated (membrane form) and soluble when unpalmitoylated. The soluble form of the protein binds vitamin A. Defects in RPE65 causes autosomal dominant retinitis pigmentosa and/or Leber congenital amaurosis type 2. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIP |
| WB:1:1,000-1:2,000 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | All-trans-retinyl-palmitate hydrolase antibody LCA 2 antibody LCA2 antibody Leber congenital amaurosis antibody mRPE 65 antibody mRPE65 antibody p63 antibody rd 12 antibody rd12 antibody Retinal pigment epithelium specific 61 kDa protein antibody Retinal pigment epithelium specific 65 kDa protein antibody Retinal pigment epithelium specific protein antibody Retinal pigment epithelium specific protein 65kDa antibody Retinal pigment epithelium-specific 65 kDa protein antibody Retinitis pigmentosa 20 antibody Retinoid isomerohydrolase antibody Retinol isomerase antibody RP 20 antibody RP20 antibody RPE 65 antibody RPE65 antibody RPE65_HUMAN antibody sRPE 65 antibody sRPE65 antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cytoplasm. Cell membrane. |
Application
Fig1: Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.| Positive Control | Mouse eyeball, rat eyeball cells lysates. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
