Application
Fig1: Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution. Positive control: Lane 1: 293T Lane 2:MCF-7Application
Fig2: ICC staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CTCF in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CTCF in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CTCF antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CTCF antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CTCF antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CTCF antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CTCF antibody. Counter stained with hematoxylin.Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CTCF antibody. Counter stained with hematoxylin.Application
Fig11: Flow cytometric analysis of 293T cells with CTCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig12: Western blot analysis of CTCF on Zebrafish tissue lysates using anti-CTCF antibody at 1/200 dilution.Application
Fig13: Western blot analysis of CTCF on hybrid fish (crucian-carp) brain tissue lysate using anti-CTCF antibody at 1/500 dilution.| Product Name | CTCF [JM10-61] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 11 zinc finger protein antibody 11 zinc finger transcriptional repressor antibody 11-zinc finger protein antibody CCCTC binding factor (zinc finger protein) antibody CCCTC binding factor antibody CCCTC-binding factor antibody Ctcf antibody CTCF_HUMAN antibody CTCFL paralog antibody MRD21 antibody Transcriptional repressor CTCF antibody |
| Product description | CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2 class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid beta-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development. CTCF is ubiquitously expressed and localized to the nucleus. During terminal differentiation, CTCF is negatively regulated by differential phosphorylation and also by decreases in CTCF mRNA and protein expression. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 IP:1:50-1:100 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | 11 zinc finger protein antibody 11 zinc finger transcriptional repressor antibody 11-zinc finger protein antibody CCCTC binding factor (zinc finger protein) antibody CCCTC binding factor antibody CCCTC-binding factor antibody Ctcf antibody CTCF_HUMAN antibody CTCFL paralog antibody MRD21 antibody Transcriptional repressor CTCF antibody |
|---|---|
| Molecular Weight(MW) | 140/83 kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution. Positive control: Lane 1: 293T Lane 2:MCF-7
Application
Fig2: ICC staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CTCF in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CTCF in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Application
Fig11: Flow cytometric analysis of 293T cells with CTCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig12: Western blot analysis of CTCF on Zebrafish tissue lysates using anti-CTCF antibody at 1/200 dilution.
Application
Fig13: Western blot analysis of CTCF on hybrid fish (crucian-carp) brain tissue lysate using anti-CTCF antibody at 1/500 dilution.| Positive Control | Hela, 293T, MCF-7, NIH-3T3, human liver tissue, human breast carcinoma tissue, human kidney tissue, human endometrium tissue, mouse colon tissue, mouse stomach tissue, Zebrafish tissue lysates. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 IP:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
