Application
Fig1: Western blot analysis of MyoD1 on Hela cells lysates using anti-MyoD1 antibody at 1/1,000 dilution.Application
Fig2: Flow cytometric analysis of Hela cells with MyoD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | MyoD1 [JM10-72] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bHLHc1 antibody Class C basic helix-loop-helix protein 1 antibody MYF 3 antibody Myf-3 antibody MYF3 antibody Myoblast determination protein 1 antibody Myod 1 antibody MYOD antibody MYOD1 antibody MYOD1_HUMAN antibody Myogenic differentiation 1 antibody Myogenic factor 3 antibody Myogenic factor MYF 3 antibody Myogenin D1 antibody PUM antibody |
| Product description | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either MyoD or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:1,000-1:5,000 IP:1:10-1:50 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | bHLHc1 antibody Class C basic helix-loop-helix protein 1 antibody MYF 3 antibody Myf-3 antibody MYF3 antibody Myoblast determination protein 1 antibody Myod 1 antibody MYOD antibody MYOD1 antibody MYOD1_HUMAN antibody Myogenic differentiation 1 antibody Myogenic factor 3 antibody Myogenic factor MYF 3 antibody Myogenin D1 antibody PUM antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P15172 |
|---|
Application
Fig1: Western blot analysis of MyoD1 on Hela cells lysates using anti-MyoD1 antibody at 1/1,000 dilution.
Application
Fig2: Flow cytometric analysis of Hela cells with MyoD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 IP:1:10-1:50 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
