Application
Fig1: Western blot analysis of Calcineurin A on different cells lysates using anti-Calcineurin A antibody at 1/500 dilution. Positive control： Lane 1: Hlea Lane 2: 293 Lane 3: A431Application
Fig2: Flow cytometric analysis of Hela cells with Calcineurin A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Calcineurin A [JA64-11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha isoform formerly PPP2B antibody Calcineurin A alpha antibody Calcineurin A1 antibody CalcineurinA antibody Calmodulin dependent calcineurin A subunit alpha isoform antibody Calmodulin-dependent calcineurin A subunit alpha isoform antibody CALN antibody CALNA 1 antibody CALNA antibody CALNA1 antibody CAM PRP catalytic subunit antibody CAM-PRP catalytic subunit antibody CCN 1 antibody CCN1 antibody CNA 1 antibody CNA alpha antibody CNA antibody CNA1 antibody PP2BA_HUMAN antibody PPP2B antibody Ppp3ca antibody Protein phosphatase 2B catalytic subunit antibody Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody Protein phosphatase 3 catalytic subunit alpha isozyme antibody Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody |
| Product description | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-Aα, PP2B-Aβ and PP2B-Aγ. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Alpha isoform formerly PPP2B antibody
Calcineurin A alpha antibody Calcineurin A1 antibody CalcineurinA antibody Calmodulin dependent calcineurin A subunit alpha isoform antibody Calmodulin-dependent calcineurin A subunit alpha isoform antibody CALN antibody CALNA 1 antibody CALNA antibody CALNA1 antibody CAM PRP catalytic subunit antibody CAM-PRP catalytic subunit antibody CCN 1 antibody CCN1 antibody CNA 1 antibody CNA alpha antibody CNA antibody CNA1 antibody PP2BA_HUMAN antibody PPP2B antibody Ppp3ca antibody Protein phosphatase 2B catalytic subunit antibody Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody Protein phosphatase 3 catalytic subunit alpha isozyme antibody Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody |
|---|---|
| Molecular Weight(MW) | 59 kD |
| Cellular Localization | Cell membrane, Cytoplasm. |
Application
Fig1: Western blot analysis of Calcineurin A on different cells lysates using anti-Calcineurin A antibody at 1/500 dilution. Positive control： Lane 1: Hlea Lane 2: 293 Lane 3: A431
Application
Fig2: Flow cytometric analysis of Hela cells with Calcineurin A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Hela, 293, A431. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
