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MUC2 [JA10-92]

Catalog Number:OM628488
OM628488
Discount Price
OM628488-100ul
1~2 Week
100ul
OM628488-50ul
50ul
OM628488-20ul
20ul
1~2 Week
Product Profile
Product Name MUC2 [JA10-92]
Antibody Type Primary Antibodies
Antigen Alias Intestinal mucin 2 antibody Intestinal mucin-2 antibody MLP antibody MUC 2 antibody MUC-2 antibody Muc2 antibody MUC2_HUMAN antibody Mucin 2 antibody Mucin 2 intestinal antibody Mucin 2 intestinal/tracheal antibody Mucin 2 oligomeric mucus/gel forming antibody Mucin 2 precursor antibody Mucin 2 tracheal antibody Mucin like protein antibody Mucin-2 antibody Mucin2 antibody SMUC antibody
Product description The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major com-ponent of various mucus gels and is broadly expressed in normal and tumor cells.
Immunogen recombinant protein
Key Feature
Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications WBICC/IFIHCFC
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity HumanRat
Concentration 1mg/ml
Target Information
Alternative Names Intestinal mucin 2 antibody Intestinal mucin-2 antibody MLP antibody MUC 2 antibody MUC-2 antibody Muc2 antibody MUC2_HUMAN antibody Mucin 2 antibody Mucin 2 intestinal antibody Mucin 2 intestinal/tracheal antibody Mucin 2 oligomeric mucus/gel forming antibody Mucin 2 precursor antibody Mucin 2 tracheal antibody Mucin like protein antibody Mucin-2 antibody Mucin2 antibody SMUC antibody
Molecular Weight(MW) 540 kDa
Cellular Localization Secreted.
Database Links
SwissProt ID Q02817 Q62635
Application

Application

Fig1: ICC staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig2: ICC staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig3: ICC staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.

Application

Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.

Application

Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.

Application

Fig7: Immunohistochemical analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.

Application

Fig8: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.

Application

Fig9: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.

Application

Fig10: Flow cytometric analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Positive Control HepG2, SW480, Hela, human breast cancer tissue, human colon cancer tissue, human tonsil tissue, rat skeletal muscle tissue, rat intestine tissue, rat brain tissue.
Application Notes WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Additional Information
Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
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