Application
Fig1: Western blot analysis of BNIP3 on different cells lysates using anti- BNIP3 antibody at 1/500 dilution. Positive control： Line 1: Human skeletal muscle Line 2: Mouse kidneyApplication
Fig2: ICC staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining BNIP3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining BNIP3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.| Product Name | BNIP3 [JA71-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody BNIP 3 antibody BNIP3 antibody BNIP3_HUMAN antibody NIP 3 antibody NIP3 antibody |
| Product description | The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. BNIP-3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the anti-apoptosis activity of Bcl-xL. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody BNIP 3 antibody BNIP3 antibody BNIP3_HUMAN antibody NIP 3 antibody NIP3 antibody |
|---|---|
| Molecular Weight(MW) | 21/30 kDa |
| Cellular Localization | Membrane, Mitochondrion, Mitochondrion outer membrane. |
Application
Fig1: Western blot analysis of BNIP3 on different cells lysates using anti- BNIP3 antibody at 1/500 dilution. Positive control： Line 1: Human skeletal muscle Line 2: Mouse kidney
Application
Fig2: ICC staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining BNIP3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining BNIP3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BNIP3 antibody. Counter stained with hematoxylin.| Positive Control | Hela, SH-SY5Y, NIH-3T3, human skeletal muscle, mouse kidney cell lysates, human colon cancer tissue, human breast carcinoma tissue, human kidney tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
