Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.| Product Name | Vitamin D Receptor [JA11-16] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1 25 dihydroxyvitamin D3 receptor antibody 1 antibody 1,25 dihydroxyvitamin D3 receptor antibody 1,25-@dihydroxyvitamin D3 receptor antibody 25-dihydroxyvitamin D3 receptor antibody Member 1 antibody NR1I1 antibody Nuclear receptor subfamily 1 group I member 1 antibody PPP1R163 antibody Protein phosphatase 1, regulatory subunit 163 antibody VDR antibody VDR_HUMAN antibody Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody Vitamin D hormone receptor antibody Vitamin D nuclear receptor variant 1 antibody Vitamin D receptor antibody Vitamin D3 receptor antibody |
| Product description | This gene encodes the nuclear hormone receptor for vitamin D3. The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. |
| Immunogen | Recombinant protein with N terminal human Vitamin D Receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 1 25 dihydroxyvitamin D3 receptor antibody
1 antibody
1 25 dihydroxyvitamin D3 receptor antibody 1 25-@dihydroxyvitamin D3 receptor antibody 25-dihydroxyvitamin D3 receptor antibody Member 1 antibody NR1I1 antibody Nuclear receptor subfamily 1 group I member 1 antibody PPP1R163 antibody Protein phosphatase 1 regulatory subunit 163 antibody VDR antibody VDR_HUMAN antibody Vitamin D (1 25- dihydroxyvitamin D3) receptor antibody Vitamin D hormone receptor antibody Vitamin D nuclear receptor variant 1 antibody Vitamin D receptor antibody Vitamin D3 receptor antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Nucleus. |
Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Application
Fig5: Flow cytometric analysis of Vitamin D Receptor was done on Hela cells. The cells were fixed, permeabilized and stained with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.| Positive Control | Rat colon tissue, human skin tissue, mouse colon tissue, mouse skin tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
