Application
Fig1: Western blot analysis of Lin-28 on NCCIT cells lysates using anti-Lin-28 antibody at 1/500 dilution.Application
Fig2: Flow cytometric analysis of Hela cells with Lin-28 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Lin28 [JA10-63] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AL024421 antibody CSDD1 antibody CSDD2 antibody FLJ12457 antibody Lin 28 antibody Lin 28 homolog (C. elegans) antibody Lin 28 homolog A (C. elegans) antibody Lin 28 homolog A antibody Lin 28 homolog antibody Lin-28A antibody Lin28 antibody Lin28, C. elegans, homolog of, A antibody LIN28A antibody LN28A_HUMAN antibody Protein lin-28 homolog A antibody Protein lin-28 homolog B antibody RNA binding protein lin 28 antibody Tex17 antibody ZCCHC1 antibody Zinc finger CCHC domain containing 1 antibody Zinc finger CCHC domain containing protein 1 antibody Zinc finger CCHC domain-containing protein 1 antibody |
| Product description | LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein sysnthesis efficiency and binds to the growth and differentiation factor IGF-II. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPFC |
| WB:1:500-1:1,000 IP:1:10-1:50 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AL024421 antibody
CSDD1 antibody
CSDD2 antibody
FLJ12457 antibody
Lin 28 antibody
Lin 28 homolog (C. elegans) antibody
Lin 28 homolog A (C. elegans) antibody
Lin 28 homolog A antibody
Lin 28 homolog antibody
Lin-28A antibody
Lin28 antibody
Lin28 C. elegans homolog of A antibody LIN28A antibody LN28A_HUMAN antibody Protein lin-28 homolog A antibody Protein lin-28 homolog B antibody RNA binding protein lin 28 antibody Tex17 antibody ZCCHC1 antibody Zinc finger CCHC domain containing 1 antibody Zinc finger CCHC domain containing protein 1 antibody Zinc finger CCHC domain-containing protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q9H9Z2 |
|---|
Application
Fig1: Western blot analysis of Lin-28 on NCCIT cells lysates using anti-Lin-28 antibody at 1/500 dilution.
Application
Fig2: Flow cytometric analysis of Hela cells with Lin-28 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NCCIT cells lysates, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IP:1:10-1:50 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
