Application
Fig1: ICC staining IL4 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining IL4 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining IL4 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL4 antibody. Counter stained with hematoxylin.| Product Name | IL4 [JA86-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B cell growth factor 1 antibody B cell IgG differentiation factor antibody B Cell Stimulatory Factor 1 antibody B-cell stimulatory factor 1 antibody BCGF 1 antibody BCGF1 antibody Binetrakin antibody BSF-1 antibody BSF1 antibody IGG1 induction factor antibody IL 4 antibody IL-4 antibody IL4 antibody IL4_HUMAN antibody Il4e12 antibody Interleukin 4 antibody Interleukin 4 variant 2 antibody Interleukin 4, isoform 1 antibody Interleukin-4 antibody Lymphocyte stimulatory factor 1 antibody MGC79402 antibody Pitrakinra antibody |
| Product description | Interleukin-4 (IL-4), also designated B cell stimulatory factor-1, is a glycosylated cytokine secreted by activated T lymphocytes, basophils and mast cells. The secreted IL-4 protein promotes the growth and differentiation of cells that participate in immune defense by favoring such events as the expansion of the Th2 lineage relative to Th1 cells. These T helper cell subsets are defined by their pattern of cytokine secretion: Th1 cells secrete IL-2, TNFβ and IFN-γ, while Th2 cells secrete IL-4, IL-5 and IL-10. Another key immunological function of IL-4 is to induce immunoglobulin class switching. IL-4 has been shown to induce the production of IgE and enhance IgG4 secretion by B cells, but suppress the production of IgM, IgA, IgG1, IgG2, and IgG3. It has been determined that Stat6 is rapidly tyrosine phosphorylated following stimulation of IL-3 or IL-4, but is not detectably phosphorylated following stimulation with IL-2, IL-12 or erythropoietin. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | B cell growth factor 1 antibody
B cell IgG differentiation factor antibody
B Cell Stimulatory Factor 1 antibody
B-cell stimulatory factor 1 antibody
BCGF 1 antibody
BCGF1 antibody
Binetrakin antibody
BSF-1 antibody
BSF1 antibody
IGG1 induction factor antibody
IL 4 antibody
IL-4 antibody
IL4 antibody
IL4_HUMAN antibody
Il4e12 antibody
Interleukin 4 antibody
Interleukin 4 variant 2 antibody
Interleukin 4 isoform 1 antibody Interleukin-4 antibody Lymphocyte stimulatory factor 1 antibody MGC79402 antibody Pitrakinra antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P05112 |
|---|
Application
Fig1: ICC staining IL4 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining IL4 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining IL4 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IL4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL4 antibody. Counter stained with hematoxylin.| Positive Control | MCF-7,D3, Hela, human colon cancer tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
