Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | GCLC [JA08-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 6.3.2.2 antibody Gamma ECS antibody Gamma glutamylcysteine synthetase antibody Gamma-ECS antibody Gamma-glutamylcysteine synthetase antibody GCL antibody Gclc antibody GCS antibody GCS heavy chain antibody GLCL antibody GLCLC antibody Glutamate cysteine ligase catalytic subunit antibody Glutamatecysteine ligase catalytic subunit antibody GSH1_HUMAN antibody |
| Product description | The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500~1:2000 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | EC 6.3.2.2 antibody Gamma ECS antibody Gamma glutamylcysteine synthetase antibody Gamma-ECS antibody Gamma-glutamylcysteine synthetase antibody GCL antibody Gclc antibody GCS antibody GCS heavy chain antibody GLCL antibody GLCLC antibody Glutamate cysteine ligase catalytic subunit antibody Glutamatecysteine ligase catalytic subunit antibody GSH1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of Jurkat cells with GCLC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | A431, rat kidney tissue, human fetal kidney tissue, human fetal liver tissue, Jurkat. |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 FC:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
