Application
Fig1: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/500 dilution.Positive control: Lane 1: HepG2Lane2 :HelaLane3:Mouse embryonic stem cellLane 4:PC12Lane5:F9Application
Fig2: ICC staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Bax in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gall bladder tissue using anti-Bax antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cervical tissue using anti-Bax antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Bax antibody. Counter stained with hematoxylin.| Product Name | BAX |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis regulator BAX antibody BAX antibody Bax-protein antibody BAX_HUMAN antibody BAXA antibody Baxdelta2G9 antibody Baxdelta2G9omega antibody Baxdelta2omega antibody Bcl-2-like protein 4 antibody BCL2 associated X protein antibody BCL2 associated X protein omega antibody BCL2 associated X protein transcript variant delta2 antibody Bcl2-L-4 antibody BCL2L4 antibody membrane isoform alpha antibody |
| Product description | BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX. |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:200 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis regulator BAX antibody BAX antibody Bax-protein antibody BAX_HUMAN antibody BAXA antibody Baxdelta2G9 antibody Baxdelta2G9omega antibody Baxdelta2omega antibody Bcl-2-like protein 4 antibody BCL2 associated X protein antibody BCL2 associated X protein omega antibody BCL2 associated X protein transcript variant delta2 antibody Bcl2-L-4 antibody BCL2L4 antibody membrane isoform alpha antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Mitochondrion membrane, cytoplasm |
Application
Fig1: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/500 dilution.Positive control: Lane 1: HepG2Lane2 :HelaLane3:Mouse embryonic stem cellLane 4:PC12Lane5:F9
Application
Fig2: ICC staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Bax in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human gall bladder tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human cervical tissue using anti-Bax antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Bax antibody. Counter stained with hematoxylin.| Positive Control | HepG2, Hela, mouse embryonic stem cell, PC12, F9 293, human gall bladder tissue, human cervical tissue, mouse testis tissue |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:200 IHC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
