Application
Fig1: Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution.Positive control:Lane 1: HelaLane2: SW480Lane3: HCT116Lane 4: HepG2Lane5: PC12Application
Fig2: ICC staining of ERK1 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-ERK1 antibody. Counter stained with hematoxylin.| Product Name | ERK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK 1 antibody ERK antibody ERK-1 antibody ERK1 antibody ERT 2 antibody ERT2 antibody Extracellular Signal Regulated Kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 1 antibody HGNC6877 antibody HS44KDAP antibody HUMKER1A antibody Insulin Stimulated MAP2 Kinase antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 3 antibody MAP Kinase antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 3 antibody MAPK antibody MAPK1 antibody Mapk3 antibody MGC20180 antibody Microtubule Associated Protein 2 Kinase antibody Microtubule-associated protein 2 kinase antibody Mitogen Activated Protein Kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 3 antibody MK03_HUMAN antibody OTTHUMP00000174538 antibody OTTHUMP00000174541 antibody p44 ERK1 antibody p44 MAPK antibody p44-ERK1 antibody p44-MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM 3 antibody PRKM3 antibody Protein Kinase Mitogen Activated 3 antibody |
| Product description | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:200 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ERK 1 antibody ERK antibody ERK-1 antibody ERK1 antibody ERT 2 antibody ERT2 antibody Extracellular Signal Regulated Kinase 1 antibody Extracellular signal related kinase 1 antibody Extracellular signal-regulated kinase 1 antibody HGNC6877 antibody HS44KDAP antibody HUMKER1A antibody Insulin Stimulated MAP2 Kinase antibody Insulin-stimulated MAP2 kinase antibody MAP kinase 1 antibody MAP kinase 3 antibody MAP Kinase antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 3 antibody MAPK antibody MAPK1 antibody Mapk3 antibody MGC20180 antibody Microtubule Associated Protein 2 Kinase antibody Microtubule-associated protein 2 kinase antibody Mitogen Activated Protein Kinase 3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 3 antibody MK03_HUMAN antibody OTTHUMP00000174538 antibody OTTHUMP00000174541 antibody p44 ERK1 antibody p44 MAPK antibody p44-ERK1 antibody p44-MAPK antibody P44ERK1 antibody P44MAPK antibody PRKM 3 antibody PRKM3 antibody Protein Kinase Mitogen Activated 3 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Cytoplasm, nucleus |
| SwissProt ID | P27361 |
|---|
Application
Fig1: Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution.Positive control:Lane 1: HelaLane2: SW480Lane3: HCT116Lane 4: HepG2Lane5: PC12
Application
Fig2: ICC staining of ERK1 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-ERK1 antibody. Counter stained with hematoxylin.| Positive Control | Hela, SW480, HCT116, HepG2, PC12, LOVO, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:200 IHC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
