Application
Fig1: Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution. Positive control: Lane 1: Raji Lane2: HUVEC Lane3: K562Application
Fig2: ICC staining of ICAM1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of Hela?cells with ICAM1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | ICAM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen identified by monoclonal antibody BB2 antibody BB 2 antibody BB2 antibody CD 54 antibody CD_antigen=CD54 antibody CD54 antibody Cell surface glycoprotein P3.58 antibody Human rhinovirus receptor antibody ICAM 1 antibody ICAM-1 antibody ICAM1 antibody ICAM1_HUMAN antibody intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody Intercellular adhesion molecule 1 antibody Major group rhinovirus receptor antibody MALA 2 antibody MALA2 antibody MyD 10 antibody MyD10 antibody P3.58 antibody Surface antigen of activated B cells, BB2 antibody |
| Product description | ICAM-1 is a member of the immunoglobulin superfamily, the superfamily of proteins including antibodies and T-cell receptors. The structure of ICAM-1 is characterized by heavy glycosylation, and the protein’s extracellular domain is composed of multiple loops created by disulfide bridges within the protein. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. More recently, ICAM-1 has been characterized as a site for the cellular entry of human rhinovirus. ICAM-1 and soluble ICAM-1 have antagonistic effects on the tight junctions forming the blood-testis barrier, thus playing a major role in spermatogenesis. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Antigen identified by monoclonal antibody
BB2 antibody
BB 2 antibody
BB2 antibody
CD 54 antibody
CD_antigen=CD54 antibody
CD54 antibody
Cell surface glycoprotein P3.58 antibody
Human rhinovirus receptor antibody
ICAM 1 antibody
ICAM-1 antibody
ICAM1 antibody
ICAM1_HUMAN antibody
intercellular adhesion molecule 1 (CD54) human rhinovirus receptor antibody Intercellular adhesion molecule 1 antibody Major group rhinovirus receptor antibody MALA 2 antibody MALA2 antibody MyD 10 antibody MyD10 antibody P3.58 antibody Surface antigen of activated B cells BB2 antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Cell membrane, Nucleus, Mitochondrion |
| SwissProt ID | P05362 |
|---|
Application
Fig1: Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution. Positive control: Lane 1: Raji Lane2: HUVEC Lane3: K562
Application
Fig2: ICC staining of ICAM1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of Hela?cells with ICAM1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | Raji, HUVEC, K562, human tonsil tissue, Hela |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:200 IHC:1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
