Application
Fig1: Western blot analysis of ERK2 on different cell lysates using anti- ERK2 antibody at 1/2000 dilution.Positive control:Lane 1: JurkatLane 2:A431Lane 3: PC12Lane 4: NIH/3T3Lane 5: HelaLane 6: HT-29Lane 7: MCF-7Application
Fig2: ICC staining of ERK2 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERK2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ERK2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela?cells with SIRT1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | ERK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK 1 antibody ERK 2 antibody ERK-2 antibody ERK1 antibody erk1/2 antibody ERK2 antibody ERT1 antibody ERT2 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody MAPK 3 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p42-MAPK antibody PRKM 2 antibody |
| Product description | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:2,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ERK 1 antibody ERK 2 antibody ERK-2 antibody ERK1 antibody erk1/2 antibody ERK2 antibody ERT1 antibody ERT2 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody MAPK 3 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p42-MAPK antibody PRKM 2 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm, nucleus |
| SwissProt ID | P28482 |
|---|
Application
Fig1: Western blot analysis of ERK2 on different cell lysates using anti- ERK2 antibody at 1/2000 dilution.Positive control:Lane 1: JurkatLane 2:A431Lane 3: PC12Lane 4: NIH/3T3Lane 5: HelaLane 6: HT-29Lane 7: MCF-7
Application
Fig2: ICC staining of ERK2 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela?cells with SIRT1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | Jurkat, A431, PC12, NIH/3T3, Hela, HT-29, MCF-7, A549, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:200 IHC:1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
