Application
Fig1: ICC staining NFAT2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining NFAT2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NFAT2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human skeleton muscle tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Jurkat cells with NFAT2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | NFAT2 [JA81-03] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cytoplasmic 1 antibody MGC138448 antibody NF ATc antibody NF ATc1 antibody NF-ATc antibody NF-ATc1 antibody NF-ATc1.2 antibody NFAC1_HUMAN antibody NFAT 2 antibody NFAT transcription complex cytosolic component antibody NFATC 1 antibody NFATc antibody NFATc1 antibody Nuclear factor of activated T cells cytoplasmic 1 antibody Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody Nuclear factor of activated T cells cytosolic component 1 antibody nuclear factor of activated T-cells 'c' antibody Nuclear factor of activated T-cells antibody |
| Product description | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| FC:1:50-1:100 WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | cytoplasmic 1 antibody MGC138448 antibody NF ATc antibody NF ATc1 antibody NF-ATc antibody NF-ATc1 antibody NF-ATc1.2 antibody NFAC1_HUMAN antibody NFAT 2 antibody NFAT transcription complex cytosolic component antibody NFATC 1 antibody NFATc antibody NFATc1 antibody Nuclear factor of activated T cells cytoplasmic 1 antibody Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody Nuclear factor of activated T cells cytosolic component 1 antibody nuclear factor of activated T-cells 'c' antibody Nuclear factor of activated T-cells antibody |
|---|---|
| Molecular Weight(MW) | 101 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | O95644 |
|---|
Application
Fig1: ICC staining NFAT2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining NFAT2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NFAT2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human skeleton muscle tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Jurkat cells with NFAT2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | 293T, Hela, MCF-7, Jurkat, human tonsil tissue, human spleen tissue, human skeleton muscle tissue, huamn thymus tissue. |
|---|---|
| Application Notes | FC:1:50-1:100 WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
