Application
Fig1: Western blot analysis of GDNF on HepG2 cell using anti-GDNF antibody at 1/1,000 dilution.Application
Fig2: Flow cytometric analysis of SH-SY5Y cells with GDNF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | GDNF [JA93-10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Astrocyte derived trophic factor antibody Astrocyte derived trophic factor 1 antibody Astrocyte-derived trophic factor antibody ATF 1 antibody ATF 2 antibody Atf antibody ATF1 antibody ATF2 antibody gdnf antibody GDNF_HUMAN antibody Glial cell derived neurotrophic factor antibody Glial Cell Line Derived Neurotrophic Factor antibody Glial cell line-derived neurotrophic factor antibody Glial derived neurotrophic factor antibody HFB1 GDNF antibody hGDNF antibody HSCR3 antibody |
| Product description | Glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons. GDNF is a glycosylated, disulfide-bonded homodimer and is a distantly related member of the TGFβ superfamily of growth regulatory ligands. GDNF contains the seven conserved cysteine residues in the same relative spacing characteristic of all members of the TGFβ superfamily. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. On the basis of these findings, it has been suggested that GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:1,000 FC:1:50-1:200 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Astrocyte derived trophic factor antibody Astrocyte derived trophic factor 1 antibody Astrocyte-derived trophic factor antibody ATF 1 antibody ATF 2 antibody Atf antibody ATF1 antibody ATF2 antibody gdnf antibody GDNF_HUMAN antibody Glial cell derived neurotrophic factor antibody Glial Cell Line Derived Neurotrophic Factor antibody Glial cell line-derived neurotrophic factor antibody Glial derived neurotrophic factor antibody HFB1 GDNF antibody hGDNF antibody HSCR3 antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of GDNF on HepG2 cell using anti-GDNF antibody at 1/1,000 dilution.
Application
Fig2: Flow cytometric analysis of SH-SY5Y cells with GDNF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | HepG2, SH-SY5Y. |
|---|---|
| Application Notes | WB:1:500-1:1,000 FC:1:50-1:200 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
