Application
Fig1: Western blot analysis of Annexin A2 on different cells lysates using anti-Annexin A2 antibody at 1/500 dilution. Positive control： Lane 1: Hela Lane 2: Human kidney Lane 3: Murine testisApplication
Fig2: ICC staining Annexin A2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of K562 cells with Annexin A2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Annexin A2 [JA42-30] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Annexin A2 antibody Annexin II antibody Annexin II, heavy chain antibody Annexin-2 antibody ANX 2 antibody ANX2 antibody ANX2L4 antibody ANXA2 antibody ANXA2_HUMAN antibody arylsulfatase B antibody CAL1H antibody Calpactin I heavy chain antibody calpactin I heavy polypeptide (p36) antibody Calpactin I heavy polypeptide antibody Calpactin-1 heavy chain antibody chromobindin 8 antibody Chromobindin-8 antibody Epididymis secretory protein Li 270 antibody HEL S 270 antibody LIP2 antibody Lipocortin II antibody LPC2 antibody LPC2D antibody p36 antibody P36 protein antibody PAP-IV antibody Placental anticoagulant protein IV antibody Protein I antibody |
| Product description | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino-terminal region which may confer binding specificity. The interaction between these proteins and biological membranes has led to the hypothesis that they are involved in cellular trafficking processes such as endocytosis, exocytosis and cellular adhesion. Annexin I, alternatively referred to as lipocortin, has been implicated as a mediator of the anti-inflammatory response produced by glucocorticoids and as an inhibitor of cPLA2, a potent mediator of inflammation. Annexin II, also called p36, exists as a monomer or as a heterotetramer, complexed with the S-100-related protein p11. This complex is termed calpactin I. In the tetrameric form, Annexin II is an efficient substrate of PKC family and Src pp60. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCICC/IFFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 ICC:1:100-1:200: FC:1:50-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Annexin A2 antibody
Annexin II antibody
Annexin II heavy chain antibody Annexin-2 antibody ANX 2 antibody ANX2 antibody ANX2L4 antibody ANXA2 antibody ANXA2_HUMAN antibody arylsulfatase B antibody CAL1H antibody Calpactin I heavy chain antibody calpactin I heavy polypeptide (p36) antibody Calpactin I heavy polypeptide antibody Calpactin-1 heavy chain antibody chromobindin 8 antibody Chromobindin-8 antibody Epididymis secretory protein Li 270 antibody HEL S 270 antibody LIP2 antibody Lipocortin II antibody LPC2 antibody LPC2D antibody p36 antibody P36 protein antibody PAP-IV antibody Placental anticoagulant protein IV antibody Protein I antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Secreted. Melanosome. |
Application
Fig1: Western blot analysis of Annexin A2 on different cells lysates using anti-Annexin A2 antibody at 1/500 dilution. Positive control： Lane 1: Hela Lane 2: Human kidney Lane 3: Murine testis
Application
Fig2: ICC staining Annexin A2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Annexin A2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of K562 cells with Annexin A2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Hela cell lysate, human kidney tissue lysate, murine testis tissue lysate, K562, human liver tissue, human colon cancer tissue, human prostate tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 ICC:1:100-1:200: FC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
