Application
Fig1: Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.| Product Name | Clathrin heavy chain [JA53-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHC17 antibody Clathrin heavy chain antibody Clathrin heavy chain 1 antibody Clathrin heavy chain 2 antibody Clathrin heavy chain like 1 antibody Clathrin heavy chain on chromosome 17 antibody Clathrin heavy polypeptide antibody CLH 17 antibody CLH 22 antibody CLH-17 antibody CLH1_HUMAN antibody CLH17 antibody CLH22 antibody CLTC antibody CLTCL antibody CLTCL1 antibody CLTCL2 antibody CLTD antibody Hc antibody KIAA0034 antibody |
| Product description | Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are englufed by clathrin-coated vesicles. Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain binding site. The proximal segment of the Clathrin HC protein is essential for interactions between clathrin heavy chains and light chains which result in the formation of the triskelion structure. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CHC17 antibody Clathrin heavy chain antibody Clathrin heavy chain 1 antibody Clathrin heavy chain 2 antibody Clathrin heavy chain like 1 antibody Clathrin heavy chain on chromosome 17 antibody Clathrin heavy polypeptide antibody CLH 17 antibody CLH 22 antibody CLH-17 antibody CLH1_HUMAN antibody CLH17 antibody CLH22 antibody CLTC antibody CLTCL antibody CLTCL1 antibody CLTCL2 antibody CLTD antibody Hc antibody KIAA0034 antibody |
|---|---|
| Molecular Weight(MW) | 191/187 kDa |
| Cellular Localization | Cytoplasmic vesicle membrane. |
Application
Fig1: Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.| Positive Control | Hela, PC12, human adrenal gland tissue, human colon cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
