Application
Fig1: Western blot analysis of PHF10 on Hela cell lysate using anti-PHF10 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PHF10 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PHF10 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHF10 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PHF10 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PHF10 antibody. Counter stained with hematoxylin.| Product Name | PHF10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BAF45a antibody BRG1-associated factor 45a antibody FLJ10975 antibody MGC111009 antibody PHD finger protein 10 antibody PHD finger protein 10, isoform a antibody PHD zinc finger protein XAP135 antibody Phf10 antibody PHF10_HUMAN antibody XAP135 antibody |
| Product description | Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:1,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BAF45a antibody
BRG1-associated factor 45a antibody
FLJ10975 antibody
MGC111009 antibody
PHD finger protein 10 antibody
PHD finger protein 10 isoform a antibody PHD zinc finger protein XAP135 antibody Phf10 antibody PHF10_HUMAN antibody XAP135 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of PHF10 on Hela cell lysate using anti-PHF10 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PHF10 antibody. Counter stained with hematoxylin.| Positive Control | Hela, rat testis tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
