Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | NOX2/gp91phox |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMCBX2 antibody CGD antibody CGD91-phox antibody CY24B_HUMAN antibody CYBB antibody Cytochrome b 245, beta polypeptide antibody Cytochrome b(558) beta chain antibody Cytochrome b(558) subunit beta antibody Cytochrome b-245 heavy chain antibody Cytochrome b558 subunit beta antibody GP91 PHOX antibody gp91-1 antibody gp91-phox antibody GP91PHOX antibody Heme-binding membrane glycoprotein gp91phox antibody NADPH oxidase 2 antibody Neutrophil cytochrome b 91 kDa polypeptide antibody NOX2 antibody p22 phagocyte B-cytochrome antibody P91 PHOX antibody p91-PHOX antibody Superoxide-generating NADPH oxidase heavy chain subunit antibody |
| Product description | Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells. |
| Immunogen | Synthetic peptide corresponding to C-terminal human NOX2/gp91phox. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:500-1:1,000 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | AMCBX2 antibody
CGD antibody
CGD91-phox antibody
CY24B_HUMAN antibody
CYBB antibody
Cytochrome b 245 beta polypeptide antibody Cytochrome b(558) beta chain antibody Cytochrome b(558) subunit beta antibody Cytochrome b-245 heavy chain antibody Cytochrome b558 subunit beta antibody GP91 PHOX antibody gp91-1 antibody gp91-phox antibody GP91PHOX antibody Heme-binding membrane glycoprotein gp91phox antibody NADPH oxidase 2 antibody Neutrophil cytochrome b 91 kDa polypeptide antibody NOX2 antibody p22 phagocyte B-cytochrome antibody P91 PHOX antibody p91-PHOX antibody Superoxide-generating NADPH oxidase heavy chain subunit antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig9: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, Hela, MCF-7,HepG2, human tonsil tissue, human liver tissue, human spleen tissue, mouse spleen tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:500-1:1,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
