Application
Fig1: Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution. Positive control： Lane1: Mouse brain Lane2: Human fetal brainApplication
Fig2: ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Hela cells with DKK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | DKK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dickkopf 1 antibody Dickkopf 1 homolog antibody Dickkopf 1 like antibody Dickkopf homolog 1 antibody Dickkopf like protein 1 antibody Dickkopf related protein 1 antibody Dickkopf WNT signaling pathway inhibitor 1 antibody Dickkopf-1 antibody Dickkopf-related protein 1 antibody DKK 1 antibody Dkk-1 antibody Dkk1 antibody DKK1_HUMAN antibody hDkk 1 antibody hDkk-1 antibody SK antibody |
| Product description | The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Dickkopf 1 antibody Dickkopf 1 homolog antibody Dickkopf 1 like antibody Dickkopf homolog 1 antibody Dickkopf like protein 1 antibody Dickkopf related protein 1 antibody Dickkopf WNT signaling pathway inhibitor 1 antibody Dickkopf-1 antibody Dickkopf-related protein 1 antibody DKK 1 antibody Dkk-1 antibody Dkk1 antibody DKK1_HUMAN antibody hDkk 1 antibody hDkk-1 antibody SK antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution. Positive control： Lane1: Mouse brain Lane2: Human fetal brain
Application
Fig2: ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Hela cells with DKK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Mouse brain tissue lysate, human fetal brain tissue lysate, Hela, NCCIT. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
