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smooth muscle Myosin heavy chain 11 [JA03-35]

Catalog Number:OM628617
OM628617
Discount Price
OM628617-100ul
1~2 Week
100ul
OM628617-50ul
50ul
OM628617-20ul
20ul
1~2 Week
Product Profile
Product Name smooth muscle Myosin heavy chain 11 [JA03-35]
Antibody Type Primary Antibodies
Antigen Alias AAT4 antibody DKFZp686D10126 antibody DKFZp686D19237 antibody FAA4 antibody FLJ35232 antibody MGC126726 antibody MGC32963 antibody MYH 11 antibody MYH11 antibody MYH11_HUMAN antibody Myosin 11 antibody Myosin heavy chain 11 antibody Myosin heavy chain 11 smooth muscle antibody Myosin heavy chain antibody Myosin heavy chain smooth muscle isoform antibody Myosin heavy polypeptide 11 smooth muscle antibody Myosin-11 antibody SMHC antibody SMMHC antibody smooth muscle isoform antibody Smooth muscle myosin heavy chain 11 isoform SM2 antibody Smooth muscle myosin heavy chain isoform SM2 antibody
Product description Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits, which is expressed by my calcium and calmodulin-dependent phosphorylation of Myosin light chain (MLC) Myosin heavy chains, encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the Speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on th E functional activity of the muscle.
Immunogen recombinant protein
Key Feature
Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications WBIHCICC/IF
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Species Reactivity HumanRat
Concentration 1mg/ml
Target Information
Alternative Names AAT4 antibody DKFZp686D10126 antibody DKFZp686D19237 antibody FAA4 antibody FLJ35232 antibody MGC126726 antibody MGC32963 antibody MYH 11 antibody MYH11 antibody MYH11_HUMAN antibody Myosin 11 antibody Myosin heavy chain 11 antibody Myosin heavy chain 11 smooth muscle antibody Myosin heavy chain antibody Myosin heavy chain smooth muscle isoform antibody Myosin heavy polypeptide 11 smooth muscle antibody Myosin-11 antibody SMHC antibody SMMHC antibody smooth muscle isoform antibody Smooth muscle myosin heavy chain 11 isoform SM2 antibody Smooth muscle myosin heavy chain isoform SM2 antibody
Molecular Weight(MW) 227 kDa
Cellular Localization Melanosome.
Database Links
SwissProt ID P35749 Q63862
Application

Application

Fig1: ICC staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig2: ICC staining smooth muscle Myosin heavy chain 11 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Application

Fig3: Immunohistochemical analysis of paraffin-embedded human jejunum tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.

Application

Fig4: Immunohistochemical analysis of paraffin-embedded rat trachea tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.

Application

Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.

Application

Fig6: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.

Application

Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.
Positive Control 293T, Hela, rat trachea tissue, human prostate tissue, human stomach cancer tissue, human colon tissue, human jejunum tissue.
Application Notes WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Additional Information
Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
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