Application
Fig1: ICC staining ROBO1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining ROBO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ROBO1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.| Product Name | ROBO1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Deleted in U twenty twenty antibody DUTT 1 antibody DUTT1 antibody FLJ21882 antibody H Robo 1 antibody H-Robo-1 antibody hRobo 1 antibody Robo 1 antibody Robo1 antibody ROBO1_HUMAN antibody Roundabout 1 antibody Roundabout axon guidance receptor homolog 1 antibody Roundabout homolog 1 antibody Roundabout homolog1 precurser antibody Roundabout1 antibody SAX 3 antibody SAX3 antibody |
| Product description | Specific cells in the midline separate the left and right halves of the central nervous system and play many roles in guiding the growth cone behavior. In vertebrate spinal cord, insect abdominal nerves and nematodes, midline cells produce induced cues, such as nectins and slits, respectively, as attractants and repellents. respectively. These cells can serve as gatekeepers to prevent axons from passing through the midline and to guide the growth cone in response to the switching of lead cues beyond the crossing. One such gatherer, Robo, is an axon guidance receptor that defines a new subfamily of proteins from fruit flies to mammalian conserved Ig superfamily proteins. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous manner Non-intersecting axons express high levels of Robo and cross axons to express low levels of Robo and then reach midline and high levels of crossover. Robo1 and Robo2 are two human homologs of Drosophila megalopolis. Robo1 is also homologous to the C. elegans gene sax3, while Robo2 is homologous to zebrafish genes. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCIHC |
| ICC:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Deleted in U twenty twenty antibody DUTT 1 antibody DUTT1 antibody FLJ21882 antibody H Robo 1 antibody H-Robo-1 antibody hRobo 1 antibody Robo 1 antibody Robo1 antibody ROBO1_HUMAN antibody Roundabout 1 antibody Roundabout axon guidance receptor homolog 1 antibody Roundabout homolog 1 antibody Roundabout homolog1 precurser antibody Roundabout1 antibody SAX 3 antibody SAX3 antibody |
|---|---|
| Molecular Weight(MW) | 180 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: ICC staining ROBO1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining ROBO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ROBO1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.| Positive Control | Hela, MCF-7, A549, human liver cancer tissue, human breast tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue. |
|---|---|
| Application Notes | ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
