WB
Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.WB
Western blot analysis of PRTN3 on mouse marrow lysate using anti-PRTN3 antibody at 1/100 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PRTN3 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | PRTN3 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The major features of Wegener granulomatosis are necrotizing granulomatous lesions, which most often affect the upper and lower airways and are associated with vasculitis, necrotizing glomerulonephritis and pulmonary capillaritis. The antigen responsible for this disease is Proteinase 3 (PR3, P29 or myeloblastin), which is one of the antibiotic proteins of neutrophilic granules belonging to the serine protease family. It is closely related to two others: Neutrophil Elastase and azurocidin. All three genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. PR3 is a neutrophil protein which is able to cleave elastin and is involved in proliferation of human leukemia cells. PR3 is expressed specifically in immature myeloid cells and is a G-CSF-responsive protein critical to factor-independent growth. The genes for all three of the related serine protease family members are located in a cluster on the tip of the short arm of human chromosome 19. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500 IHC:1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | ACPA antibody
AGP 7 antibody
AGP7 antibody
AGP7 serine proteinase antibody
Azurophil Granule Protein 7 antibody
C ANCA antibody
C ANCA antigen antibody
C-ANCA antigen antibody
CANCA antibody
EC 3.4.21.76 antibody
Leukocyte proteinase 3 antibody
MBN antibody
MBT antibody
MBT WEGENER AUTOANTIGEN antibody
Myeloblastin antibody
Neutrophil proteinase 4 antibody
NP 4 antibody
NP-4 antibody
NP4 antibody
P29 antibody
PR 3 antibody
PR-3 antibody
PR3 antibody
Proteinase 3 antibody
Proteinase3 antibody
PRTN 3 antibody
Prtn3 antibody
PRTN3_HUMAN antibody
Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody
Serine proteinase neutrophil antibody Wegener autoantigen antibody Wegener granulomatosis autoantigen antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasmic granule, Secreted, Cell membrane, Membrane raft. |
WB
Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution.
WB
Western blot analysis of PRTN3 on mouse marrow lysate using anti-PRTN3 antibody at 1/100 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PRTN3 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
