Application
Fig1: Western blot analysis of PINK1 on different lysates using anti-PINK1 antibody at 1/200 dilution. Positive control： Lane1: A431 Lane2: Mouse testis tissue Lane3: JurkatApplication
Fig2: ICC staining PINK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PINK1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PINK1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PINK1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PINK1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-PINK1 antibody. Counter stained with hematoxylin.| Product Name | PINK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRPK antibody FLJ27236 antibody mitochondrial antibody PARK 6 antibody PARK6 antibody Phosphatase and Tensin Homolog antibody PINK 1 antibody PINK1 antibody PINK1_HUMAN antibody Protein kinase BRPK antibody PTEN induced putative kinase 1 antibody PTEN induced putative kinase protein 1 antibody PTEN-induced putative kinase protein 1 antibody Serine/threonine kinase PINK1 mitochondrial antibody Serine/threonine protein kinase PINK1 mitochondrial antibody Serine/threonine-protein kinase PINK1 antibody |
| Product description | A member of the serine / threonine protein kinase family, PTEN induced putative kinase 1 (PINK1) is a tumor suppressor. PINK1 is primarily located in mitochondria, and is ubiquitously expressed in testis, skeletal muscle, and heart tissue. at lower levels in pancreas, ovary, brain, placenta, kidney, liver, prostate and small intestine. During cellular stress PINK1 protects against mitochondrial dysfunction by inducing phosphorylation mitochondrial proteins. PINK1 mutations may give rise to different autophosphorylation activity. Mutations in the PINK1 gene (PARK6) are associated with early onset Parkinson's disease, a recessive neurodegenerative disorder characterized by resting tremor, muscular rigidity, bradykinesia and postural instability. Parkinson's disease generally involves the presence of intraneuronal accumulations of aggregated proteins (Lewy bodies) in brain neurons. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | BRPK antibody FLJ27236 antibody mitochondrial antibody PARK 6 antibody PARK6 antibody Phosphatase and Tensin Homolog antibody PINK 1 antibody PINK1 antibody PINK1_HUMAN antibody Protein kinase BRPK antibody PTEN induced putative kinase 1 antibody PTEN induced putative kinase protein 1 antibody PTEN-induced putative kinase protein 1 antibody Serine/threonine kinase PINK1 mitochondrial antibody Serine/threonine protein kinase PINK1 mitochondrial antibody Serine/threonine-protein kinase PINK1 antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Mitochondrion outer membrane. Cytoplasm. |
Application
Fig1: Western blot analysis of PINK1 on different lysates using anti-PINK1 antibody at 1/200 dilution. Positive control： Lane1: A431 Lane2: Mouse testis tissue Lane3: Jurkat
Application
Fig2: ICC staining PINK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PINK1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PINK1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PINK1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PINK1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-PINK1 antibody. Counter stained with hematoxylin.| Positive Control | A431, mouse testis tissue lysate, Jurkat, Hela, PC-3M, SK-Br-3, human breast tissue, human placenta tissue, human stomach cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
