Application
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibodyApplication
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibodyApplication
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibodyApplication
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibodyApplication
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibodyApplication
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Product Name | ATF6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating transcription factor 6 alpha antibody Activating transcription factor 6 antibody ATF 6 antibody ATF6 alpha antibody ATF6 antibody ATF6-alpha antibody ATF6A antibody ATF6A_HUMAN antibody cAMP dependent transcription factor ATF 6 alpha antibody cAMP-dependent transcription factor ATF-6 alpha antibody Cyclic AMP dependent transcription factor ATF 6 alpha antibody DKFZp686P2194 antibody ESTM49 antibody FLJ21663 antibody Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody |
| Product description | Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina. |
| Immunogen | Recombinant protein within full sequence of human ATF6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | Activating transcription factor 6 alpha antibody Activating transcription factor 6 antibody ATF 6 antibody ATF6 alpha antibody ATF6 antibody ATF6-alpha antibody ATF6A antibody ATF6A_HUMAN antibody cAMP dependent transcription factor ATF 6 alpha antibody cAMP-dependent transcription factor ATF-6 alpha antibody Cyclic AMP dependent transcription factor ATF 6 alpha antibody DKFZp686P2194 antibody ESTM49 antibody FLJ21663 antibody Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody |
|---|---|
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling. |
| SwissProt ID | P18850 |
|---|
Application
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Application
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Application
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Application
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Application
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Application
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody| Positive Control | Hela, A431, HUVEC, K562, rat brain tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
