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Fig1: Western blot analysis of Kv1.4 on rat brain (1) and mouse brain (2) tissue lysate using anti-Kv1.4 antibody at 1/1,000 dilution.
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Fig2: ICC staining Kv1.4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig3: ICC staining Kv1.4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.
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Fig6: Flow cytometric analysis of SH-SY5Y cells with Kv1.4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.