Application
Fig1: Western blot analysis of Bag3 on Hela (1) and MCF-7 (2) cell using anti-Bag3 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Bag3 antibody. Counter stained with hematoxylin.| Product Name | Bag3 [JA90-53] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BAG 3 antibody BAG family molecular chaperone regulator 3 antibody BAG-3 antibody Bag3 antibody BAG3_HUMAN antibody Bcl 2 binding protein antibody Bcl-2-associated athanogene 3 antibody Bcl-2-binding protein Bis antibody BCL2 associated athanogene 3 antibody BCL2 binding athanogene 3 antibody BIS antibody CAIR 1 antibody Docking protein CAIR 1 antibody Docking protein CAIR-1 antibody MFM6 antibody |
| Product description | The Bag family of proteins are characterized by the presence of a 45 amino acid Bag domain through which they bind with high affinity to the ATPase domain of HSP 70, thereby negatively regulating HSP 70 chaperone activity. Bag-3 (Bcl-2-associated athanogene 3), also known as BIS or CAIR-1, is a 575 amino acid protein that contains one C-terminal Bag domain and two N-terminal WW domains. Like other members of the Bag family, Bag-3 functions to inhibit the chaperone activity of HSP 70, specifically by promoting the release of HSP 70-bound substrates. Additionally, Bag-3 exhibits anti-apoptotic activity via cell cycle control, suggesting a possible role for Bag-3 in tumor progression. The gene encoding Bag-3 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:1,000-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BAG 3 antibody BAG family molecular chaperone regulator 3 antibody BAG-3 antibody Bag3 antibody BAG3_HUMAN antibody Bcl 2 binding protein antibody Bcl-2-associated athanogene 3 antibody Bcl-2-binding protein Bis antibody BCL2 associated athanogene 3 antibody BCL2 binding athanogene 3 antibody BIS antibody CAIR 1 antibody Docking protein CAIR 1 antibody Docking protein CAIR-1 antibody MFM6 antibody |
|---|---|
| Molecular Weight(MW) | 75 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | O95817 |
|---|
Application
Fig1: Western blot analysis of Bag3 on Hela (1) and MCF-7 (2) cell using anti-Bag3 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Bag3 antibody. Counter stained with hematoxylin.| Positive Control | Hela, MCF-7, human fetal skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
