Application
Fig1: Western blot analysis of CLOCK on MCF-7 (1) and PC-12 (2) cell using anti-CLOCK antibody at 1/1,000 dilution.Application
Fig2: ICC staining CLOCK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CLOCK in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CLOCK antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded 2 colon tissue using anti-CLOCK antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CLOCK antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CLOCK antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | CLOCK [JA12-33] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bHLHe8 antibody Circadian locomoter output cycles kaput protein antibody Circadian locomoter output cycles protein kaput antibody Circadian Locomotor Output Cycles Kaput antibody Circadium Locomotor Output Cycles Kaput antibody Class E basic helix-loop-helix protein 8 antibody CLOCK antibody Clock circadian regulator antibody Clock homolog antibody Clock protein antibody CLOCK_HUMAN antibody hCLOCK antibody KIAA0334 antibody |
| Product description | Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the Basic-helix-loop-helix-psp (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the clock gene have been shown to increase the length of the endogenous period and To contain a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation ability. It has been speculated that Clock may regulation circadian rhythmicity in combination with Other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:500-1:1000: ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | bHLHe8 antibody Circadian locomoter output cycles kaput protein antibody Circadian locomoter output cycles protein kaput antibody Circadian Locomotor Output Cycles Kaput antibody Circadium Locomotor Output Cycles Kaput antibody Class E basic helix-loop-helix protein 8 antibody CLOCK antibody Clock circadian regulator antibody Clock homolog antibody Clock protein antibody CLOCK_HUMAN antibody hCLOCK antibody KIAA0334 antibody |
|---|---|
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of CLOCK on MCF-7 (1) and PC-12 (2) cell using anti-CLOCK antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CLOCK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CLOCK in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CLOCK antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded 2 colon tissue using anti-CLOCK antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CLOCK antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CLOCK antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | MCF-7, PC-12, Hela, SHG-44, human fetal skeletal muscle tissue, 2 colon tissue, human colon cancer tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1000: ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
