Application
Fig1: Western blot analysis of Peroxiredoxin 3 on different cell lysate using anti-Peroxiredoxin 3 antibody at 1/1,000 dilution. Positive control： Lane1: Human liver Lane2: MCF-7 Lane3: A431Application
Fig2: ICC staining Peroxiredoxin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Peroxiredoxin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Peroxiredoxin 3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of MCF-7 cells with Peroxiredoxin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Peroxiredoxin 3 [JA53-21] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antioxidant protein 1 antibody AOP 1 antibody AOP-1 antibody AOP1 antibody HBC189 antibody MER5 antibody MGC104387 antibody MGC24293 antibody mitochondrial antibody peroxiredoxin 3 antibody Peroxiredoxin III antibody Peroxiredoxin-3 antibody PRDX3 antibody PRDX3_HUMAN antibody PRO1748 antibody Protein MER5 homolog antibody PRX III antibody Prx-III antibody PRX3 antibody SP 22 antibody SP-22 antibody SP22 antibody Thioredoxin dependent peroxide reductase mitochondrial antibody Thioredoxin-dependent peroxide reductase antibody |
| Product description | The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis. The human PRX IV gene is abundantly expressed in many tissues. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIPFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Antioxidant protein 1 antibody AOP 1 antibody AOP-1 antibody AOP1 antibody HBC189 antibody MER5 antibody MGC104387 antibody MGC24293 antibody mitochondrial antibody peroxiredoxin 3 antibody Peroxiredoxin III antibody Peroxiredoxin-3 antibody PRDX3 antibody PRDX3_HUMAN antibody PRO1748 antibody Protein MER5 homolog antibody PRX III antibody Prx-III antibody PRX3 antibody SP 22 antibody SP-22 antibody SP22 antibody Thioredoxin dependent peroxide reductase mitochondrial antibody Thioredoxin-dependent peroxide reductase antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Mitochondrion. |
| SwissProt ID | P30048 |
|---|
Application
Fig1: Western blot analysis of Peroxiredoxin 3 on different cell lysate using anti-Peroxiredoxin 3 antibody at 1/1,000 dilution. Positive control： Lane1: Human liver Lane2: MCF-7 Lane3: A431
Application
Fig2: ICC staining Peroxiredoxin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Peroxiredoxin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Peroxiredoxin 3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of MCF-7 cells with Peroxiredoxin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Human liver tissue lysate, MCF-7, A431, Hela, HepG2, human liver cancer, human kidney. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
