Application
Fig1: Western blot analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.Application
Fig2: ICC staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Product Name | NLRC3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CARD15-like antibody CARD15-like protein antibody Caterpiller 16.2 antibody Caterpiller protein 16.2 antibody CLR16.2 antibody FLJ00348 antibody NLR family CARD domain containing 3 antibody Nlrc3 antibody NLRC3_HUMAN antibody NOD-like receptor C3 antibody NOD3 antibody NOD3 protein antibody Nucleotide-binding oligomerization domain protein 3 antibody Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 antibody nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 antibody Protein NLRC3 antibody |
| Product description | NLRC3, short for NOD-like receptor family CARD domain containing 3, is an intracellular protein that plays a role in the immune system. It was previously known as nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 (NOD3) and CLR16.2. NLRC3 inhibits the activity of T cells. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CARD15-like antibody
CARD15-like protein antibody
Caterpiller 16.2 antibody
Caterpiller protein 16.2 antibody
CLR16.2 antibody
FLJ00348 antibody
NLR family CARD domain containing 3 antibody
Nlrc3 antibody
NLRC3_HUMAN antibody
NOD-like receptor C3 antibody
NOD3 antibody
NOD3 protein antibody
Nucleotide-binding oligomerization domain protein 3 antibody
Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 antibody
nucleotide-binding oligomerization domain leucine rich repeat and CARD domain containing 3 antibody Protein NLRC3 antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Cytoplasm. Cytoskeleton. |
Application
Fig1: Western blot analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution.
Application
Fig2: ICC staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Human thymus tissue lysates, HUVEC, MCF-7, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
