Application
Fig1: Western blot analysis of ROCK2 on Raji (1) and SiHa (2) cell lysates using anti-ROCK2 antibody at 1/500 dilution.Application
Fig2: ICC staining ROCK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ROCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.| Product Name | ROCK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | coiled-coil-containing protein kinase 2 antibody KIAA0619 antibody p164 ROCK 2 antibody p164 ROCK-2 antibody Rho associated coiled coil containing protein kinase 2 antibody Rho associated protein kinase 2 antibody Rho associated, coiled coil containing protein kinase II antibody Rho kinase 2 antibody Rho-associated antibody Rho-associated protein kinase 2 antibody ROCK 2 antibody Rock II antibody Rock2 antibody ROCK2_HUMAN antibody Rock2m antibody ROK alpha antibody ROKalpha antibody |
| Product description | Rho, the Ras-related small GTPase, is responsible for the regulation of Actin-based cytoskeletal structures, including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been de-scribed, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka) for Rho-associated coil-containing protein kinase. Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | coiled-coil-containing protein kinase 2 antibody
KIAA0619 antibody
p164 ROCK 2 antibody
p164 ROCK-2 antibody
Rho associated coiled coil containing protein kinase 2 antibody
Rho associated protein kinase 2 antibody
Rho associated coiled coil containing protein kinase II antibody Rho kinase 2 antibody Rho-associated antibody Rho-associated protein kinase 2 antibody ROCK 2 antibody Rock II antibody Rock2 antibody ROCK2_HUMAN antibody Rock2m antibody ROK alpha antibody ROKalpha antibody |
|---|---|
| Molecular Weight(MW) | 161 kDa |
| Cellular Localization | Plasma membrane. Cytoskeleton. Nucleus. |
Application
Fig1: Western blot analysis of ROCK2 on Raji (1) and SiHa (2) cell lysates using anti-ROCK2 antibody at 1/500 dilution.
Application
Fig2: ICC staining ROCK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ROCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.| Positive Control | Raji, siHa, Hela, HepG2, rat brain tissue, human liver tissue, human colon cancer tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
